• Recombinant Human Novel Coronavirus Spike glycoprotein(S), partial (Active)

    In Stock
    • 货号:
      CSB-YP3324GMY1
    • 规格:
      ¥11424
    • 图片:
      • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
        Predicted band size: 38.2 kDa
        Observed band size:
        (a) 68 kDa before EndoH Digestion
        (b) 38 kDa after EndoH Digestion

      • Activity
        Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.

        Biological Activity Assay

      • Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml.

        Biological Activity Assay

      • Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A0GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 13.48-19.50 ng/ml.

        Biological Activity Assay

      • SARS-CoV-2 Spike protein RBD his/sumostar tag (CSB-YP3324GMY1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 100 nM as detected by LSPR Assay.

        Biological Activity Assay

      • SARS-CoV-2 Spike protein RBD His/Sumostar Tag (CSB-YP3324GMY1) captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with an affinity constant of 28.2nM as detected by LSPR Assay. Biological Activity Assay

    • 其他:

    产品详情

    • 纯度:
      Greater than 90% as determined by SDS-PAGE.
    • 生物活性:
      ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml.③Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A0GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 13.48-19.50 ng/ml.④SARS-CoV-2 Spike protein RBD his/sumostar tag (CSB-YP3324GMY1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 100 nM as detected by LSPR Assay.⑤SARS-CoV-2 Spike protein RBD His/Sumostar Tag (CSB-YP3324GMY1) captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with an affinity constant of 28.2nM as detected by LSPR Assay.
    • Uniprot No.:
    • 种属:
      Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
    • 蛋白长度:
      Partial
    • 来源:
      Yeast
    • 分子量:
      38.2 kDa
    • 表达区域:
      319-541aa
    • 氨基酸序列
      RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
    • 蛋白标签:
      N-terminal 6xHis-sumostar-tagged
    • 产品提供形式:
      Lyophilized powder
      Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
    • 缓冲液:
      Lyophilized from 20?mM?Tris-HCl,?0.5 M?NaCl, 6% Trehalose, pH 8.0
    • 复溶:
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
    • 储存条件:
      Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
    • 保质期:
      The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
      Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
    • 货期:
      3-7 business days
    • 注意事项:
      Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
    • 产品描述:

      A cDNA ORF corresponding to the 319-541 AA of human SARS-CoV-2 spike glycoprotein (S) was expressed with an N-terminal 6xHis-sumostar-tag in the yeast. The product is the partial-length recombinant human novel coronavirus (SARS-CoV-2) S protein. Its purity is greater than 90% determined by SDS-PAGE. The gel showed a band with a molecular weight of approximately 68 kDa, which was confirmed to be due to glycosylation after EndoH digestion. More importantly, its biological activity was validated by the functional ELISA and LSPR Assay. And it may be used in the research area of microbiology. This recombinant SARS-CoV-2 S protein is in-stock now.

      SARS-CoV-2 S protein is composed of S1 subunit and S2 subunit. During viral infection, the receptor-binding domain (RBD) of the S1 subunit is responsible for the recognition and binding of host receptor ACE2, while the S2 subunit mediates viral cell membrane fusion. The SARS-CoV-2-S1-RBD/ACE2 interaction mediates viral entry into the target cells. The cleavage by TMPRSS2 primes the S protein for cell entry.

    • Datasheet & COA:
      Please contact us to get it.

    引用文献



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